As scientists continue to learn about the biology of aging, the role of epigenetics in regulating the aging process has become evident. This webinar discusses some of the epigenetic mechanisms known to be involved in aging and highlights the tools Active Motif has developed to aid in their study. Topics covered in the webinar will include: Overview of the roles of chromatin accessibility and DN...

Pet owners are looking for alternatives to annual or even triennial vaccination and research has shown that positive titers are strong proof of immunity. Veterinarians that offer titer testing affirm their position as experts in animal care and give their teams a sense of pride in what they do. In this BioGal sponsored lecture, Bash Halow, CVPM, LVT, and author for Veterinary Economics, AAHA Trends, and DVM360...

A subset of COVID-19 patients experience cytokine storm syndrom with elevated levels of inflammatory cytokines in the lungs, leading to tissue damage. To assess these cytokines, researchers can use multiplex assays, like BioLegend's LEGENDplex™, to quantitate the factors made in response to SARS-CoV-2 infection. Understanding what drives severe tissue damage during COVID-19 infection will better help us u...

Endogenous peroxidase (HRP) present in tissue sections can result in background when using an HRP detection system if this activity is not quenched. Methods for quenching this endogenous enzyme activity include treating with BLOXALL Endogenous Enzyme Blocking Solution or with various preparations of hydrogen peroxide. In this video tutoria...

Vector® VIP and ImmPACT™ VIP substrates fr4om Vector Laboratories both produce intense, violet reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector VIP and ImmPACT VIP substrates also provide excellent color contrast in pigmented tissues such as melanoma. With the aid of imaging systems and software, the sp...

The ability to precisely edit and change any part of an organism’s genome has long been sought by scientists, and today, we are closer to that goal than ever before. With the discovery of the CRISPR Cas9 system, scientists are now able to effortlessly and efficiently knock-out or knock-in any gene of interest. Given its ease of...

Host cell proteins (HCPs) are process-related impurities, expressed by the host cell used for production of biopharmaceutical proteins. While the majority of these HCPs are removed during the purification process, there are still residual HCP amounts remaining in the distributed products, with unknown effects on protein stability or immunogenicity in the patients....

Engineering complex biological systems is not straightforward. To this end, synthetic biologists often need to explore a combinatorial design space to achieve the best performance. In this webinar, we will discuss strategies for designing and assembling combinatorial DNA libraries to optimize or develop novel metabolic pathways, genetic circuits, or microbial strains. We will also introduce our comprehensive combinatorial DNA library services, which can greatly accelerate the build phase of your development cycle and facilitate your engineering of nov...

In this webinar, Kelly Lundsten, Business Segment Manager for Advanced Cytometry at BioLegend, describes the properties of spectral cytometers such as the Cytek Aurora and how to set up panels for multicolor flow cytometry. She then describes the real world application of panel creation on the instrument by showing data generated from a 26-color B cell lymphopoiesis panel. Content of this webinar: - Spectral cytometry - Fluorophore development - Panel design - 26 color B-cell lymphopoi...

Nonspecific binding interactions, sample variation, background noise, and matrix interference are all common problems encountered when performing ELISA testing. ICT offers a range of diluent options designed to help overcome these issues. ICT’s Sample Diluents are used to dilute ELISA test samples so they read within the functional range of the assay. Our Assay Diluents are added to all wells of the plate to equalize any differences between the sample matrix and the diluent used to generate the standard curve. The right sample diluent can aid in...