Ki-67/MIB1 has been established as the reference marker for assessing cellular proliferation in tumour cells. The antibody identifies actively dividing cells at all stages of the cell cycle (late G1, S, M and G2 phases), but does not recognize cells in G0 phase.In diagnostic histopathology, Ki-67 has been used as a marker for cell proliferation of solid tumors and some hematological malignancies. A correlation has been demonstrated between Ki-67 index and the histopathological grade of neoplasms.
Instructions for Use
Immunohistochemical staining of standard formalin-fixed paraffin sections Deparaffinize and rehydrate according to standard procedures. Heat induced epitope retrival (HIER) is required. For immunohistochemical detection different techniques can be used: indirect immunoenzyme labeling with a secondary antibody conjugate, biotin/(strept)avidin-based detection, soluble enzyme immune complex or polymer-based detection. The antibody can be adapted for use on automated staining instruments.
Product Specifications
| Artikelnummer |
DIA-670-P05 |
| Spezifität |
Ki-67/MIB1
|
| Spezies-Reaktivität |
Human
|
| Immunogen |
Fusionsprotein
|
| Wirtsspezies |
Maus
|
| Isotyp |
IgG1
|
| Klon |
Ki-67P
|
| Klonalität (Mono-/Polyklonal) |
monoklonal
|
| Anwendung |
Immunfluoreszenz, Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe), Western Blot
|
| Konjugation |
unkonjugiert
|
| Verdünnung |
Histo-/Zytochemie 1:100 – 1:200
|
| Format |
0,05% NaN3, 2% BSA, gereinigter Antikörper (aus Kulturüberstand), in PBS (pH 7,4), Lyophilisat
|
| Produktlinie / Thema |
Neurobiologie, Tumormarker/-biologie
|
| Zweckbestimmung |
für In-vitro-Diagnostik
|
| Temperatur - Lagerung |
2-8°C
|
| Temperatur - Transport |
bei Raumtemperatur
|
| Suchcode |
DIA670, FFPE, Ki-67P, Paraffin
|
| Hersteller / Marke |
dianova
|
| Uniprot_ID |
P46013
|
| Gene_ID |
4288
|
| Alias |
Antigen Identified By Monoclonal Antibody Ki-67, Ki-67 Protein, Ki67P, KIA, MIB-1, MKI67, Proliferation marker protein Ki-67
|
Intented use / regulatory status: For in Vitro Diagnostic Use (Europe) / For Research Use only (All other countries) |
