Anti-BRAF V600E (Hu), ms clone HA01
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Artikelname: | Anti-BRAF V600E (Hu), ms clone HA01 |
Artikelnummer: | DNA-DIA-HA01 |
Hersteller Artikelnummer: | DNA-DIA-HA01 |
Alternativnummer: | DIA-HA01,DNA-DIA-HA01 |
Hersteller: | dianova |
Wirt: | Mouse |
Kategorie: | Antikörper |
Applikation: | IHC |
Spezies Reaktivität: | Human |
Immunogen: | Synthetic peptide, amino acid sequence CKPIIIGHHAYGD |
Alternative Synonym: | BRAF, BRAF V600E |
Regulatory Status: RUO |
Clone HA01 for BRAF V600E HistologyClone HA01 reacts specifically with the BRAF V600E point mutation in tissue sections from routine formalin-fixed paraffin-embedded human tumors (IHC FFPE). This antibody has been validated for the identification of BRAF V600E positive cells in diverse human tumor tissues with an additional focus on neuropathological tissues selected according to WHO guidelines.
BRAF V600E as a Driver Mutation in CancerBRAF V600E is a well-established oncogenic driver mutation with broad clinical implications. Since first studies of BRAF mutations in cancers 2002 (1), BRAF V600E has evolved into a critical diagnostic, prognostic, and predictive tumor marker. BRAF has been shown to be mutated in various types of cancers including malignant melanoma (~50%), papillary thyroid cancer (~45%), colorectal cancers (~10%) and has also been identified in ovarian, breast, and lung cancers (1,2,3). Several primary CNS neoplasms harbor a BRAF V600E mutation, e.g. several pediatric low-grade gliomas (4,5): ~60% of pleomorphic xanthoastrocytomas (PXA), 20–60% of gangliogliomas (GG), and roughly 10% of pilocytic astrocytomas.
BRAF – FunctionPhysiologically, BRAF acts a serine-threonine protein kinase and is a member of the RAF kinase family playing an important role in the RAS-RAF-MAPK signaling pathway, which regulates cell survival, proliferation and differentiation (6). 90% of oncogenic mutations in the BRAF gene are a single V600E substitution within the kinase domain and result in sustained kinase activity, constitutively activating the MAPK signaling pathway which leads to carcinogenesis (7,8).
References
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Klonalität: | Monoclonal |
Klon-Bezeichnung: | HA01 |
Isotyp: | IgG2b |
UniProt: | P15056 |
Formulierung: | 0.05% NaN3, 2% BSA, in PBS (pH 7.4), lyophilisate, purified antibody (from culture supernatant) |
Lagerung: | 2-8°C |
Target-Kategorie: | BRAF V600E |
Antibody Type: | Monoclonal Antibody |
Application Verdünnung: | Histo-/Cytochemistry 1:100 |