Exosome Isolation

Purest EV isolation with ExoQuick ULTRA from SBI

Exosomes are formed by lymphocytes, dendritic cells, nerve cells and tumor cells, among others, as transport vesicles and for the expulsion (exocytosis) of cell components. They play an important role in cellular communication and acquired immunity. Viruses use exosomes for transport and camouflage. Exosomes have been the focus of research in cell communication and gene regulation for several years and are being investigated as therapeutic options in the treatment of cancer and autoimmune diseases.

Our partner SBI System Biosciences supports your research with a complete workflow from exosome isolation to quanitification and labeling.

Purest Exosome Isolation with ExoQuick ULTRA

SBI offers a portfolio of products to simplify high-quality exosome isolation from many types of biofluids. Choose from SBI's ExoQuick® family of reagents for ultracentrifugation-free isolation.

The revolutionary new ExoQuick® ULTRA is SBI's most advanced, cleanest method for isolating Extracellular Vesicles, resulting in 420X more EVs than ultracentrifugation.

Your benefits at a glance:

> Even cleaner - reduces carry-over of albumins by 75% and immunoglobulins by 40% more than other methods, including ultracentrifugation and competitors’ kits

> Higher yields - isolate more EVs per normalized input volume than ultracentrifugation and competitors’ kits

> Better biomarker detection - see what you’ve been missing when you increase the sensitivity of EV biomarker detection by up to 11-fold (CD9) and 8-fold (CD81)

> Fast - requires less than 20-minutes of hands-on time, including a convenient column-based format for clean-up

Fig.: ExoQuick ULTRA delivers high yields of clean exosomes. (A) A coomassie blue-stained protein gel comparing the protein content of exosome preps isolated using different methods shows only a few, defined protein bands in the ExoQuick ULTRA lane compared to the other methods. (B) Western blotting of the gel shows that the ExoQuick ULTRA prep contains the highest levels of exosome-specific markers CD9, CD81, and Hsp70 and the lowest levels of the carryover proteins albumin and IgGH.

Not sure which ExoQuick is right for you? Choose based on your application:

APPLICATION	PRODUCT	CATALOG #
Purest EV isolation from plasma, serum, and ascites fluid from tissue culture media	ExoQuick ULTRA ExoQuick-TC ULTRA	SBI-EQULTRA-20A-1 SBI-EQULTRA-20TC-1
General purpose EV isolation from plasma, serum, and ascites fluid from tissue culture media	ExoQuick and ExoQuick-TC	SBI-EXOQ20A-1 SBI-EXOTC50A-1
EV isolation for pre-clinical / in vivo studies	ExoQuick-CG	SBI-EXOCG50A-1
EV isolation that removes contaminating lipoprotein particles from plasma or serum	ExoQuick-LP	SBI-EXOLP5A-1
EV isolation that includes a de-fibrinating plasma step prior to isolation	ExoQuick Plasma Prep with Thrombin	SBI-EXOQ5TM-1

And for researchers looking to isolate exosomes from cultured cells, use Exo-FBS (Art. Nr. SBI-EXO-FBS-50A-1 / SBI-EXO-FBS-250A-1) to avoid contaminating your preps with bovine exosomes.