QuickSwitch™

Peptide Screening Kit from MBL Int.

MBL MBL International offers novel solutions for screening MHC binding peptides and creating custom MHC tetramers. Functional screening of peptides for MHC class I binding is essential for vaccine development and immune system monitoring. With QuitchSwitch™ from MBL, a 90-minute test system is available that allows the experimental evaluation of the MHC binding ability of candidate peptides. This is particularly important for the screening of immunogenic peptides of infectious agents or cancer neoantigens.

QuickSwitch™ Peptide Screening Kit is a fresh, new technology that allows for analysis of many peptides in an easy and ready to use high throughput format. There is no need for optimizing prior to use. One 96 well plate allows for accurate peptide-MHC binding affinity results that can be gathered in one day.

Applications of the QuickSwitch™ Peptide Screening Kit:

High throughput MHC monomer/peptide screening in a 96 well plate assay
Evaluate immune potentiators or vaccine efficacy
Neo-epitope screening for further downstream T cell recognition and activation assays
Determine peptide affinity for specific MHC Class I alleles
Target discovery of new peptides for immunotherapy and drug discovery

Example: Discovery and Validation of Lung Adenocarcinoma Heterogeneity

Lung adenocarcinoma is a type of cancer that exhibits significant variation in terms of its biological characteristics. To examine this variations Deng, Yulan, et al. (2024) analyzed 58 lung adenocarcinoma patients via machine learning, single-cell RNA sequencing (scRNA-seq), and whole-exome sequencing. They evaluated the peptides of interest for their effectiveness in peptide exchange and to generate tetramers using the QuickSwitch™ Quant HLA-A*11:01 Tetramer Kit-PE. The flow cytometry analysis was conducted to examine the binding of neoantigen-associated MHC tetramers and the levels of intracellular IFN-γ concentrations. The findings offer crucial insights into the diversity of lung adenocarcinoma, indicating promising avenues for targeted therapies in the early stages of the disease.

Article Number	Product Description
MBL-TB-7300-KM	QuickSwitch™ HLA-A*02:01 Peptide Screening Kit
MBL-TB-7400-K1	QuickSwitch™ H-2 Kb Tetramer Kit-PE
MBL-TB-7502-K1	QuickSwitch™ Quant HLA-DRB1*04:01 Tetramer Kit-PE