Mobicol Mini Columns

Incredibly versatile chromatography columns

The purification of biomolecules is an essential part of the daily laboratory routine. Protein purification is vital for the specification of the function, structural studies, and biochemical assays. Purified antibodies are essential for many research applications. High purity DNA and RNA is a prerequisite for most molecular biological applications, including enzymatic nucleic acid digestion, transformation, RT-(q)PCR, and NGS library preparation.

MoBiTec, as a part of BIOZOL, offers a variety of chromatography columns for the purification of nucleic acids, peptides, proteins, and antibodies, which make clean-up protocols easier and faster.

Mobicols – Versatile Mini Columns

Mobicols are incredibly versatile mini columns (up to 800 μl) that are an indispensable addition to every lab.
Mobicols can be used both as gravity-flow column (Mobicol Gravi) or as spin column (Mobicol Spin). They are available as empty columns or pre-filled with matrix, e.g., Sephadex® G-50 resin, different Sephacryl® resins, or silica-based resins. Luer-Lock adapters and filters with different diameters and pore sizes are offered as accessories to customize the columns to your specific needs.

Shared Features of Mobicol Gravi & Mobicol Spin Columns:

Fast and simple purification of biomolecules like nucleic acids, peptides, proteins, and other compounds
Useful for size exclusion, affinity, or ion exchange chromatography
Empty columns - fill in the matrix of choice
Also available pre-filled with different matrices
Ideal for small sample volumes
Plugs included for relocking
Filters available with 10 μm, 35 μm, and 90 μm pore sizes
Materials: polypropylene (column corpus), polyethylene (filters)
Autoclavable at 120 °C (with inserted filter at 110 °C for 10 minutes)

Additional Feature Mobicol Gravi:

Luer-Lock cap which is compatible to Luer-Lock syringes or other chromatography systems

Additional Feature Mobicol Spin:

Can be used with a standard microcentrifuge (can be centrifuged in most 1.5 ml or 2 ml collection tubes)

Empty Gravity-Flow Columns

Column chromatography can be performed with automated systems which use a pump to force solvent through a packed column at a set flow rate, or can be run by gravity-flow. The gravity method is less expensive, the user has more control, and can make adjustments during the run.

Mobicol Gravi columns are developed for performing size exclusion, affinity, or ion exchange chromatography by gravity-flow. By using the supplied Luer-Lock cap, there is no limitation in volume, i.e., it is possible to apply small or large volumes of sample or buffer. Using the Luer-Lock cap, a syringe can be connected to the top of the Mobicol Gravi column as a reservoir for sample or buffer. To enable a connection to larger reservoirs, an additional Luer-Lock adapter is available to attach chromatography tubing to the top cap. This even enables a safe connection from a pump to the inlet of a Mobicol Gravi column. Luer-Lock connections for the bottom, a One-Way Stopcock and various filters are also offered to customize the columns to specific needs.

Art. N°	Product description
M1002	Mobicol Gravi Classic with Luer-Lock and screw cap, bottom plug, without filters, 50 pcs
M1050	Mobicol Gravi Fixed with Luer-Lock and screw cap, fixed outlet plug, without filters, 50 pcs
M105035F	Mobicol Gravi Fixed with Luer-Lock and screw cap, fixed outlet plug and inserted, large 35 µm filter, 50 pcs

Empty Spin Columns - Speed-Up your Purification with a Microcentrifuge

The Mobicol Spin Columns are developed as fast and convenient routine tool for clean-up of biomolecules by spinning.

They can be used without matrix (e.g., to extract trypsinated proteins out of a gel slice for Mass Spec analysis) or filled with a matrix of your choice, e.g., for performing size exclusion or affinity chromatography. Independent of the preferred chromatography, the handling is easy: load the sample on the column, spin, and collect the flow-through. For collection, the Mobicol Spin column must be placed in a 1.5 ml or 2 ml microtube before centrifugation.

Compared to gravitation chromatography, spinning is faster.

Art. N°	Product description
M1003	Mobicol Spin Classic with screw cap, bottom plug, without filters, 50 pcs
M1053	Mobicol Spin Fixed with screw cap, fixed outlet plug, without filters, 50 pcs
M105010S	Mobicol Spin Fixed with screw cap, fixed outlet plug and inserted small 10 µm filter, 50 pcs
M105210S	Mobicol Spin Fixed with screw cap, fixed outlet plug and inserted large 10 µm filter, 50 pcs
M1002S	Mobicol Spin Classic with screw cap, bottom plug and large 10 µm filter, 100 pcs

Pre-filled Mobicols

MoBiTec offers different pre-filled mini columns:

Spin columns for purification of nucleic acids by size exclusion chromatography
Spin columns for purifications of proteins by affinity chromatography
Mini columns (for spinning or gravity-flow) with enzymatically active matrices (immobilized enzymes)

The pre-packed mini column is closed with a screw cap and contains a specific matrix. The outlet of the mini column is a fixed snap-off plug. Prior to the first centrifugation step, it has to be removed by bending. The reverse side of the plug can be used for relocking.

Features