Streptavidin Coated PCR-Tubes and Microplates
for DNA-assisted immunoassays
Newest epitope detection methods as immuno PCR assays, or DNA-assisted immunoassays combine the high specifity of antibody binding with the enormous amplification capacity of the polymerase chain reaction (PCR). Affinity probes with conjugated amplifiable oligonucleotides allow proteins to be detected at the level of single molecules or molecule complexes.
Together with our partner Biomat, specialst for surface modifications, we provide you with Streptavidin coated PCR tubes and microplates, which enable binding of biotinylated molecule complexes directly in the well for further PCR amplification.
Streptavidin coated surfaces offer a powerful and universal instrument for binding any biotinylated molecule (Proteins, Peptides, Polysaccharides, Oligonucleotides, DNA fragments etc.). Streptavidin is a tetrameric protein (M.W. 60 kDa) with very high affinity for biotin (Ka=10- 15 M); the bond is the strongest known non-covalent biological interaction.
The streptavidin-biotin bonding main features are:
- stability
- specificity
- affinity
This feature make this coating suitable for special applications of molecules which do not offer reliable bonding by passive adsorption or adsorb in a unfavorable orientation.
Biomats streptavidin coated PCR products were applied and cited in the work of Wu et al., who profiled surface proteins on individual exosomes.
Biomat streptavidin coated PCR tubes and microplates:
Streptavidin Coated PCR Tubes |
|
Streptavidin PCR Plates, non-skirted |
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BMT-PCR96020STF-SA5/100 |
Streptavidin PCR Plates, semi-skirted |
BMT-PCR96030STF-SA5/100 |
Streptavidin PCR Plates, skirted |
Biomat also offers PCR tubes and microplates coated with Protein A, Protein G, Protein A/G, Malemide, and carboxylate.
Should you be interested in other modifications, please contact the BIOZOL-team via or inquiry form.
Related Topics:
>> Webinar: An introduction to PCR
>> News: DNA/RNA Shield - Keep your samples safe!
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