Immunofluorescence

Autofluorescence Quenching with Vector TrueVIEW

Vector Laboratories is the market leader in labeling and detection reagents for immunohistochemistry, immunofluorescence, and bioconjugation with 40 years of experience. As a specialist in immunohistochemistry and immunofluorescence, Vector offers you a comprehensive range of kits and reagents for every step in your IHC workflow or IF workflow.

Vector® TrueVIEW® Autofluorescence Quenching Kit

Immunofluorescence (IF) is a powerful but simple method for visualizing protein expression in tissues or cells using fluorophore-conjugated antibodies. Interfering background signal, such as autofluorescence, which is fluorescence inherent or endogenous to tissue, cell, or due to certain fixatives, can hamper your ability to accurately distinguish real target antigen expression. Most methods for reduction of tissue autofluorescence act primarily on lipofuscin granules, and are not broadly effective against the most common sources of autofluorescence.

Vector TrueVIEW Autofluorescence Quenching Kit provides a novel way to remove unwanted fluorescence in tissue sections due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin. This unique formulation binds and effectively quenches the autofluorescent elements in even the most problematic tissues, such as kidney, spleen and pancreas. The use of the TrueVIEW quenching reagent leads to significant enhancement in overall signal-to-noise in most immunofluorescence assays.